A prototype of gene-chip based on electrical detection
碩士 === 國立中央大學 === 電機工程研究所 === 92 === In this research, we make comb-shape electrodes using semiconductor process. One pair of electrodes is two electrodes, and the distance between them is several micro-meter. Electrodes are like capacitance structure of VLSI. The surface was treated by surface chem...
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ndltd-TW-092NCU054420552015-10-13T13:04:43Z http://ndltd.ncl.edu.tw/handle/84979746600486090103 A prototype of gene-chip based on electrical detection 電子式基因序列偵測晶片之原型 Bo-Rong Liang 梁柏榮 碩士 國立中央大學 電機工程研究所 92 In this research, we make comb-shape electrodes using semiconductor process. One pair of electrodes is two electrodes, and the distance between them is several micro-meter. Electrodes are like capacitance structure of VLSI. The surface was treated by surface chemical modification and was immobilized with single strand oligonuceotide, then carry out hybridization. After the hybridization, the top-end of DNA was bond with gold nanoparticle. The silver-enhancer and gold muiltilayer deposition can be avoided if the process was used. Furthermore, the good detection was discovered and only use of UV exposure without E-beam lithography. There are some disadvantages of tradition detection methods, for example, fluorescence signal decay with time, and human body was hurt by radioactive labeling. The research avoid disadvantages of traditional detection methods, the detection was implemented by DC measurement and AC measurement on micro-electrodes. After the binding of gold nanoparticle, the change of admittance value was discovered. The detection of hybridization can be represented by the admittance value. Hybridization detection is the key technique of gene chip, the detection method of the research can be applied on gene expression, and quantification of expressed genes, differentistion of expression genes, mutations, identification of sequence, evaluation of specific DNA-binding proteins or molecules. Jang-Zern Tsai Yue-ming Hsin 蔡章仁 辛裕明 2004 學位論文 ; thesis 93 zh-TW |
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碩士 === 國立中央大學 === 電機工程研究所 === 92 === In this research, we make comb-shape electrodes using semiconductor process. One pair of electrodes is two electrodes, and the distance between them is several micro-meter. Electrodes are like capacitance structure of VLSI. The surface was treated by surface chemical modification and was immobilized with single strand oligonuceotide, then carry out hybridization. After the hybridization, the top-end of DNA was bond with gold nanoparticle. The silver-enhancer and gold muiltilayer deposition can be avoided if the process was used. Furthermore, the good detection was discovered and only use of UV exposure without E-beam lithography.
There are some disadvantages of tradition detection methods, for example, fluorescence signal decay with time, and human body was hurt by radioactive labeling. The research avoid disadvantages of traditional detection methods, the detection was implemented by DC measurement and AC measurement on micro-electrodes. After the binding of gold nanoparticle, the change of admittance value was discovered. The detection of hybridization can be represented by the admittance value.
Hybridization detection is the key technique of gene chip, the detection method of the research can be applied on gene expression, and quantification of expressed genes, differentistion of expression genes, mutations, identification of sequence, evaluation of specific DNA-binding proteins or molecules.
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author2 |
Jang-Zern Tsai |
author_facet |
Jang-Zern Tsai Bo-Rong Liang 梁柏榮 |
author |
Bo-Rong Liang 梁柏榮 |
spellingShingle |
Bo-Rong Liang 梁柏榮 A prototype of gene-chip based on electrical detection |
author_sort |
Bo-Rong Liang |
title |
A prototype of gene-chip based on electrical detection |
title_short |
A prototype of gene-chip based on electrical detection |
title_full |
A prototype of gene-chip based on electrical detection |
title_fullStr |
A prototype of gene-chip based on electrical detection |
title_full_unstemmed |
A prototype of gene-chip based on electrical detection |
title_sort |
prototype of gene-chip based on electrical detection |
publishDate |
2004 |
url |
http://ndltd.ncl.edu.tw/handle/84979746600486090103 |
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