Studies on the Preparation of Ankaflavin and Its Induced Apoptosis Effect
碩士 === 國立臺灣大學 === 農業化學研究所 === 92 === Monascus pigments were traditionally used as natural food colorants. Recently, many bioactive components of those pigments were discovered. Last year, one of Monascus pigments, ankaflavin, with selective toxicity was found. It was toxic to human cancer cell lines...
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ndltd-TW-092NTU054060162016-06-10T04:16:15Z http://ndltd.ncl.edu.tw/handle/42190053580410197402 Studies on the Preparation of Ankaflavin and Its Induced Apoptosis Effect Ankaflavin之製備與其誘導細胞凋亡之研究 Chen-Ying Ho 何貞瑩 碩士 國立臺灣大學 農業化學研究所 92 Monascus pigments were traditionally used as natural food colorants. Recently, many bioactive components of those pigments were discovered. Last year, one of Monascus pigments, ankaflavin, with selective toxicity was found. It was toxic to human cancer cell lines Hep G2 and A549 in vitro. Apoptosis was suggested as the most possible mechanism involved. In this study, an efficient method for ankaflavin preparation from commercial red rice as subject was estabilished. Pure ankaflavin was obtained after the following steps: a) extraction (hexanes as extraction solvent); b) silica gel column separation (hexanes/ethyl acetate 9:1 v/v as elution solution); c) n-hexane precipitation. The results of western blot and the cell morphology after ankaflavin treatment suggest that ankaflavin could induce cell apoptosis during M phase. 蘇南維 2004 學位論文 ; thesis 54 zh-TW |
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碩士 === 國立臺灣大學 === 農業化學研究所 === 92 === Monascus pigments were traditionally used as natural food colorants. Recently, many bioactive components of those pigments were discovered. Last year, one of Monascus pigments, ankaflavin, with selective toxicity was found. It was toxic to human cancer cell lines Hep G2 and A549 in vitro. Apoptosis was suggested as the most possible mechanism involved.
In this study, an efficient method for ankaflavin preparation from commercial red rice as subject was estabilished. Pure ankaflavin was obtained after the following steps: a) extraction (hexanes as extraction solvent); b) silica gel column separation (hexanes/ethyl acetate 9:1 v/v as elution solution); c) n-hexane precipitation. The results of western blot and the cell morphology after ankaflavin treatment suggest that ankaflavin could induce cell apoptosis during M phase.
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author2 |
蘇南維 |
author_facet |
蘇南維 Chen-Ying Ho 何貞瑩 |
author |
Chen-Ying Ho 何貞瑩 |
spellingShingle |
Chen-Ying Ho 何貞瑩 Studies on the Preparation of Ankaflavin and Its Induced Apoptosis Effect |
author_sort |
Chen-Ying Ho |
title |
Studies on the Preparation of Ankaflavin and Its Induced Apoptosis Effect |
title_short |
Studies on the Preparation of Ankaflavin and Its Induced Apoptosis Effect |
title_full |
Studies on the Preparation of Ankaflavin and Its Induced Apoptosis Effect |
title_fullStr |
Studies on the Preparation of Ankaflavin and Its Induced Apoptosis Effect |
title_full_unstemmed |
Studies on the Preparation of Ankaflavin and Its Induced Apoptosis Effect |
title_sort |
studies on the preparation of ankaflavin and its induced apoptosis effect |
publishDate |
2004 |
url |
http://ndltd.ncl.edu.tw/handle/42190053580410197402 |
work_keys_str_mv |
AT chenyingho studiesonthepreparationofankaflavinanditsinducedapoptosiseffect AT hézhēnyíng studiesonthepreparationofankaflavinanditsinducedapoptosiseffect AT chenyingho ankaflavinzhīzhìbèiyǔqíyòudǎoxìbāodiāowángzhīyánjiū AT hézhēnyíng ankaflavinzhīzhìbèiyǔqíyòudǎoxìbāodiāowángzhīyánjiū |
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1718300788935098368 |