The antioxidative capacity and antimutagenicity of phytochemicals

• Main Authors: Hsiao-Wei Huang, 黃恔瑋
• Other Authors: Pi-Yu Chao
• Format: Others
• Language: zh-TW
• Published: 2004
• Online Access: http://ndltd.ncl.edu.tw/handle/52600091422509035361

碩士 === 中國文化大學 === 生活應用科學研究所 === 92 === Abstract The aim of this study was to evaluate the antioxidative capacity and antimutagenicity of three phytochemicals, including chlorophyll and their derivatives, flavonols and anthocyanins and acid hydrolysates of leafy sweet potato. The antioxidantive substances included porphyrins, flavonols and anthocyanins were determinated. The antioxidative substances of acid hydrolysates of leafy sweet potato were also determined. The flavonols such as myricetin, quercetin, morin and kaempferol, and anthocyanins such as cyanidin and malvidin were determined. The antioxidative capacity was evaluated by the Trolox Equivalent Antioxidant Capacity (TEAC) assay and by the inhibition percentage of conjugated diene formation in linolenic acid emulsion autoxidation system. The substances, such as chlorophyllin (CHL), pheophytin a (Phe a), pheophytin b (Phe b), quercetin, cyanidin and acid hydrolysates of leafy sweet potato were evaluated their antimutagenicity by Ames test. In results, the porphyrins contents were significantly rich in Taoyuan 2. The flavonols, such as morin and quercetin were significantly rich in Ipomoea batatas (L.)(red). The anthocyanins only existed in Ipomoea batatas (L.) (red). In TEAC assary, at 7.5μM and 100μg/ mL, the antioxidative capacity of flavonols, anthocyanins and acid hydrolysates of leafy sweet potato were more than 90%. Excepting CHL, the antioxidative capacity of chlorophyll and their derivatives were less than 50%. In general, the antioxidative capacity of chlorophyll and their derivatives were much less than that of flavonols and anthocyanins. In Ames test, CHL, Phe a, Phe b, cyanidin, quercetin and acid hydrolysates of leafy sweet potato had antimutagenicity effect. In S. typhimurium TA98 system, Phe a, Phe b and quercetin had the best inhibition percentage. In TA100 system, Phe a and cyanidin had the best inhibition percentage. In TA98 and TA100 systems, the various acid hydrolysates of leafy sweet potato had antimutagenicity effect, and the inhibition percentage of Taoyuan 2 was better than that of Ipomoea batatas (L.)(red). The TEAC percentage of samples treated with acid hydrolysates of leafy sweet potato was negative correlated with porphyrins but was positive correlated with flavonols and anthocyanins. In TA98 and TA100 systems, the antimutagenicity of samples treated with acid hydrolysates of Ipomoea batatas (L.)(red) was positive correlated with cyanidin while the antimutagenicity of samples treated with acid hydrolysates of Taoyuan 2 was positive correlated with Chl a. The TEAC percentage of CRCs and cyanidin were negative correlated with the antimutagenicity of TA98 while the TEAC percentage of CRCs, cyanidin and acid hydrolysates of Ipomoea batatas (L.)(red) were positive correlated with the antimutagenicity of TA100. In conclusion, the phytochemicals including chlorophyll and their derivatives, flavonols and anthocyanins, and acid hydrolysates of leafy sweet potato had antioxidative capacity and antimutagenicity, but there was no identical correlation. Keywords: Chlorophyll, Flavonols, Anthocyanins, Phytochemicals, Antioxidative, TEAC, Ames test