Apply molecular biology technique to identify Actinomycetes in the ChengChinhu reservoir and evaluate the influence of the nitrogen and phosphorus source.

• Main Authors: Wei-Yen Hsu, 徐尉晏
• Other Authors: Ming-Muh Kao
• Format: Others
• Language: zh-TW
• Published: 2005
• Online Access: http://ndltd.ncl.edu.tw/handle/81264844389373054843

碩士 === 國立成功大學 === 環境工程學系碩博士班 === 93 === 　Odor problems in the drinking water have caused people’s higher concern and murmur recently. This research try to investigate about the musty/earthy odors were generated by Actinomycete in the Chengchinghu reservoir collocate the 2k factor design with different nitrogen and phosphorous adding to estimate the main effect factor about the odor-producing. In the other hand, we also used the tools of molecular biology technique to identify the Actinomycetes were isolated from the water and sediments of this reservoir and establish the phylogenetic tree further. 　In the odor-producing experiments, the nutrient was prepared according to highest and lowest value from the Chengchinghu reservoir in the past years. We used the ammoniam-nitrogen、nitrate-nitrogen and nitrite-nitrogen to simulate the nitrogen existence and the total phosphate represented the phosphorous concentration in the reservoir. The results of odor-producing indicate the concentration of 2-MIB and Geosmin with different Actinomycetes were between 0.27 to 42.9 ppt and 0.67 to 18.31 ppt respectively. We also find the concentration of 2-MIB and Geosmin are both very low in all experimental condition and the concentration of Geosmin was significant lower the 2-MIB. If we compared different tested Actinomycetes isolated from this reservoir, we can find the S2 had the highest odor-producing value. The results of 2k factor design showed that the main effect factors of Geosmin and 2-MIB were combined the complex effects of ammonium-nitrogen, nitrate-nitrogen and nitrite-nitrogen. The influence degree was different with dissimilar characteristics of bacteria. 　We used the molecular biology techniques to identify the isolated Actinomycetes and the results indicated all of them are Streptomyces.S1 and S4 are parts of S.thermocarboxudus；S2 and S5 are parts of S.afghaniensis：and S2 belongs to S.maritimus. If we summarized the above-mentioned results to assess the feasibility of tools of molecular biology techniques, we will find the applications of these tools were practicable of environmental samples.