The effects of sucrose concentration and gas exchange restriction on anthocyanin biosynthesis and key enzyme gene expression in Vitis vinifera cell suspension cultures

• Main Authors: shao wei ming, 邵韋銘
• Other Authors: wang jian guo
• Format: Others
• Language: zh-TW
• Published: 2007
• Online Access: http://ndltd.ncl.edu.tw/handle/02931282080669765380

碩士 === 亞洲大學 === 生物科技學系碩士班 === 94 === The aims of this thesis are to investigate the mechanism of anthocyanin biosynthesis under different sucrose level, plant growth regulators or gas restricted from which the expression of gene in anthocyanin biosynthesis were detected. Plant cell suspension was cultured in Gamborg B5 medium. The result showed that, high levels of sucrose enhance the production of anthocyanin. There is a trade-off between growth rate and production of anthocyanin, and it actually influences by cell culture condition. Meanwhile, gas restriction obviously suppresses anthocyanin production and growth simultaneously. On the gene expression of key enzyme in anthocyanin biosynthesis, the comparison bettwen the white surface grape cell, and the red surface grape cell showed that the gene expression of CHS2 increased up to 14.76 fold. Meanwhile, under gas restriction the expression of UFGT in red grape cell increased up to 11.11 fold. Consequently, gas restriction would cause CHS down regulated, implying the activity of UFGT increased to keep up the anthocyanin production. After stop the gas restriction, the production of anthocyanin reached the maximum level at the second week of incubation. On anthocyanin biosynthesis gene expression, the flods of the expression of CHS was proportional to the incubation time. On the other hand, the flods of the expression of Chi (chalcon isomerase)、F3’H ( flavanone 3-hydroxylase)、Dfr (dihydroflavonol 4-reductase) was proportionate inversely. In the second week of incubation, the expression of UFGT suddenly increased, but declined in the third week. The amount of anthocyanin production was parallel with the expression of UFGT. It might conclude that the gene expression of UFGT is critical for anthocyanin biosynthesis.