AFM-Based Nanolithography and Detection of DNA Hybridization Reactions at the Nanoscale
碩士 === 國立中山大學 === 化學系研究所 === 95 === High-resolution lattice periodicity images of a variety of well-defined surfaces, including graphite, mica, and Au(111), validated the good stability of our atomic force microscope (AFM) system. Combining self-assembled monolayer (SAM) and AFM technology, we demon...
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ndltd-TW-095NSYS50650222019-05-15T20:22:42Z http://ndltd.ncl.edu.tw/handle/gtyknx AFM-Based Nanolithography and Detection of DNA Hybridization Reactions at the Nanoscale 原子力顯微鏡之奈米圖案顯影與核酸雜化反應探討 Shu-ting Lo 羅淑婷 碩士 國立中山大學 化學系研究所 95 High-resolution lattice periodicity images of a variety of well-defined surfaces, including graphite, mica, and Au(111), validated the good stability of our atomic force microscope (AFM) system. Combining self-assembled monolayer (SAM) and AFM technology, we demonstrated the capabilities of pattern fabrication as well as modification of surface functionality. AFM-based nanolithography operating conditions, such as scan rate, deflection setpoint, and number of scan were studied to obtain the optimized quality of the fabricated patterns. Thiolated-DNA probe molecules could be patterned at a nanometer scale on a gold substrate. However, we found that the surface coverage began to drop notably with the probe length (number of DNA bases). Therefore, the displaced DNA molecules during nanoshaving were reversibly adsorbed, and patterning became unreliable. We were unsuccessful in detecting the subsequent hybridization reactions at these nanopatterns from AFM measurements. To realize the DNA hybridization, further studies on the incubation temperature, probe length and even DNA sequences are required to demonstrate that this AFM-based gene diagnostic method is truly operational. Chao-ming Chiang 蔣昭明 2007 學位論文 ; thesis 104 zh-TW |
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碩士 === 國立中山大學 === 化學系研究所 === 95 === High-resolution lattice periodicity images of a variety of well-defined surfaces, including graphite, mica, and Au(111), validated the good stability of our atomic force microscope (AFM) system. Combining self-assembled monolayer (SAM) and AFM technology, we demonstrated the capabilities of pattern fabrication as well as modification of surface functionality. AFM-based nanolithography operating conditions, such as scan rate, deflection setpoint, and number of scan were studied to obtain the optimized quality of the fabricated patterns. Thiolated-DNA probe molecules could be patterned at a nanometer scale on a gold substrate. However, we found that the surface coverage began to drop notably with the probe length (number of DNA bases). Therefore, the displaced DNA molecules during nanoshaving were reversibly adsorbed, and patterning became unreliable. We were unsuccessful in detecting the subsequent hybridization reactions at these nanopatterns from AFM measurements. To realize the DNA hybridization, further studies on the incubation temperature, probe length and even DNA sequences are required to demonstrate that this AFM-based gene diagnostic method is truly operational.
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author2 |
Chao-ming Chiang |
author_facet |
Chao-ming Chiang Shu-ting Lo 羅淑婷 |
author |
Shu-ting Lo 羅淑婷 |
spellingShingle |
Shu-ting Lo 羅淑婷 AFM-Based Nanolithography and Detection of DNA Hybridization Reactions at the Nanoscale |
author_sort |
Shu-ting Lo |
title |
AFM-Based Nanolithography and Detection of DNA Hybridization Reactions at the Nanoscale |
title_short |
AFM-Based Nanolithography and Detection of DNA Hybridization Reactions at the Nanoscale |
title_full |
AFM-Based Nanolithography and Detection of DNA Hybridization Reactions at the Nanoscale |
title_fullStr |
AFM-Based Nanolithography and Detection of DNA Hybridization Reactions at the Nanoscale |
title_full_unstemmed |
AFM-Based Nanolithography and Detection of DNA Hybridization Reactions at the Nanoscale |
title_sort |
afm-based nanolithography and detection of dna hybridization reactions at the nanoscale |
publishDate |
2007 |
url |
http://ndltd.ncl.edu.tw/handle/gtyknx |
work_keys_str_mv |
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