AFM-Based Nanolithography and Detection of DNA Hybridization Reactions at the Nanoscale

碩士 === 國立中山大學 === 化學系研究所 === 95 === High-resolution lattice periodicity images of a variety of well-defined surfaces, including graphite, mica, and Au(111), validated the good stability of our atomic force microscope (AFM) system. Combining self-assembled monolayer (SAM) and AFM technology, we demon...

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Main Authors: Shu-ting Lo, 羅淑婷
Other Authors: Chao-ming Chiang
Format: Others
Language:zh-TW
Published: 2007
Online Access:http://ndltd.ncl.edu.tw/handle/gtyknx
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spelling ndltd-TW-095NSYS50650222019-05-15T20:22:42Z http://ndltd.ncl.edu.tw/handle/gtyknx AFM-Based Nanolithography and Detection of DNA Hybridization Reactions at the Nanoscale 原子力顯微鏡之奈米圖案顯影與核酸雜化反應探討 Shu-ting Lo 羅淑婷 碩士 國立中山大學 化學系研究所 95 High-resolution lattice periodicity images of a variety of well-defined surfaces, including graphite, mica, and Au(111), validated the good stability of our atomic force microscope (AFM) system. Combining self-assembled monolayer (SAM) and AFM technology, we demonstrated the capabilities of pattern fabrication as well as modification of surface functionality. AFM-based nanolithography operating conditions, such as scan rate, deflection setpoint, and number of scan were studied to obtain the optimized quality of the fabricated patterns. Thiolated-DNA probe molecules could be patterned at a nanometer scale on a gold substrate. However, we found that the surface coverage began to drop notably with the probe length (number of DNA bases). Therefore, the displaced DNA molecules during nanoshaving were reversibly adsorbed, and patterning became unreliable. We were unsuccessful in detecting the subsequent hybridization reactions at these nanopatterns from AFM measurements. To realize the DNA hybridization, further studies on the incubation temperature, probe length and even DNA sequences are required to demonstrate that this AFM-based gene diagnostic method is truly operational. Chao-ming Chiang 蔣昭明 2007 學位論文 ; thesis 104 zh-TW
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description 碩士 === 國立中山大學 === 化學系研究所 === 95 === High-resolution lattice periodicity images of a variety of well-defined surfaces, including graphite, mica, and Au(111), validated the good stability of our atomic force microscope (AFM) system. Combining self-assembled monolayer (SAM) and AFM technology, we demonstrated the capabilities of pattern fabrication as well as modification of surface functionality. AFM-based nanolithography operating conditions, such as scan rate, deflection setpoint, and number of scan were studied to obtain the optimized quality of the fabricated patterns. Thiolated-DNA probe molecules could be patterned at a nanometer scale on a gold substrate. However, we found that the surface coverage began to drop notably with the probe length (number of DNA bases). Therefore, the displaced DNA molecules during nanoshaving were reversibly adsorbed, and patterning became unreliable. We were unsuccessful in detecting the subsequent hybridization reactions at these nanopatterns from AFM measurements. To realize the DNA hybridization, further studies on the incubation temperature, probe length and even DNA sequences are required to demonstrate that this AFM-based gene diagnostic method is truly operational.
author2 Chao-ming Chiang
author_facet Chao-ming Chiang
Shu-ting Lo
羅淑婷
author Shu-ting Lo
羅淑婷
spellingShingle Shu-ting Lo
羅淑婷
AFM-Based Nanolithography and Detection of DNA Hybridization Reactions at the Nanoscale
author_sort Shu-ting Lo
title AFM-Based Nanolithography and Detection of DNA Hybridization Reactions at the Nanoscale
title_short AFM-Based Nanolithography and Detection of DNA Hybridization Reactions at the Nanoscale
title_full AFM-Based Nanolithography and Detection of DNA Hybridization Reactions at the Nanoscale
title_fullStr AFM-Based Nanolithography and Detection of DNA Hybridization Reactions at the Nanoscale
title_full_unstemmed AFM-Based Nanolithography and Detection of DNA Hybridization Reactions at the Nanoscale
title_sort afm-based nanolithography and detection of dna hybridization reactions at the nanoscale
publishDate 2007
url http://ndltd.ncl.edu.tw/handle/gtyknx
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