Effect of nanolized black soybean【Glycine max (L.) Merr.】on learning and memory ability and immune function in senescence accelerated mice

• Main Authors: Yo-Giao Lin, 林郁喬
• Other Authors: none
• Format: Others
• Language: zh-TW
• Published: 2006
• Online Access: http://ndltd.ncl.edu.tw/handle/86379701926449277576

碩士 === 靜宜大學 === 食品營養研究所 === 95 === Glycine max (L.) Merr. may decrease the damage of reaction oxygen species (ROS) because the antioxidant capacity of isoflavone, saponin, anthocyanin and vit E components. However, whether the nano black soybean powder could be easier to absorb, enhance the physiological function, then promote the antioxidant system and slow the aging process are still unknown. The purpose of this study was to examine the DPPH scavenging ability of nano and non-nano black soybeans, and also evaluated their effects on the learning and memory ability, and antioxidant status in senescence-accelerated-prone mice (SAMP8). In vivo experiment, 6-month-old male SAMP8 mice were fed with three different diets for 12 weeks: casein diet group (the control group), casein diet supplemented with nano black soybean and non-nano black soybean. Senescence-accelerated-resistant mice (SAMR1) was used for the normal control group and fed with casein diet. Body weight, food intake, aging score, single-trial passive avoidance and active shuttle avoidance test were performed. The malondiadehyde (MDA) concentration, superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px) activities of liver, hippocampus, striatum and cortex, the -amyloid protein (A) deposition of brain, histological examination of fatty liver and serum biological values were examined after sacarificed. The motility and concentration of spermatozoa, the histopathological evaluation of dead spermatids of cauda epididymis, and splenic proliferation were also evaluated. The results showed that the total isoflavone content in nano black soybean was higher than non-nano black soybean, whereas, the fatty acid content did not differ among two black soybeans. Nano black soybean showed better DPPH scavenging ability than the non-nano black soybean. In vivo study, SAMR1 group and the SAMP8 mice fed with nano black soybean showed significantly better learning and memory ability, and lower aging scores, fatty liver scores, A deposition of brain, MDA concentration of liver and brain, the serum triglyceride and GPT concentration. The SOD, CAT and GSH-Px activity of the brain and liver, the serum HDL-C concentration in the SAMR1 and nano black soybean group were significantly higher than the control and no-nano black soybean groups. The SAMR1 group performed significantly better sperm quality than the SAMP8 control. The motility and concentration of spermatozoa in mice fed with nano black soybean were significantly higher than the control and no-nano black soybean groups, while the mean dead spermatids of cauda epididymis was significantly lower. The T cell and B cell proliferation, IL-2 and IFN-γ concentration of SAMR1 and nano black soybean groups were significantly higher than the control and non-nano black soybean groups, whereas the IL-4 concentration of nano black soybean group was significantly lower than the SAMP8 control group. In summary, we conclude that SAMP8 showed senescence accelerated characteristic. The supplements of nano black soybean could improve the learning and memory ability, reduce the lipid peroxidation and brain pathological changes, and also promote the antioxidative defense system, sperm quality and immune responses. Moreover, the effects were better than the non-nano black soybean group. However, the mechanisms of the effects of nano black soybean is still unknown and need further study.