| Summary: | 碩士 === 台南科技大學 === 生活應用科學研究所 === 95 === Three experiments of different malachite green (MG) (0.1, 0.5 or 1.0 mg/L) waterborne exposure were associated with different reared density (60, 30 or 15 fish/200 L) or environment (sea, brackish or fresh water) to investigate the accumulative concentration of MG and leucomalachite green (LMG) when the waterborne exposure, or residual concentration of MG and LMG after transferred promptly into clean water in tilapia.
1. This experiment focused on the fish was exposed to different MG levels (0.1, 0.5 and 1 mg/L) for 24 h. The result shown that the accumulative concentration of muscular MG from any treatment was significant increased with the concentration and time of MG through a waterborne exposure. The accumulative concentration of muscular MG reached the highest at 8th h during the waterborne exposure. Then the residual concentration of muscular MG was reduced follows reared time. The muscular MG residues of fish muscle were below 1μg / kg when the fish were reared up to 16th, 40th and 56th day, and the muscular LMG residues were 40th , 64th and 80th day.
2. This experiment focused on three different reared densities (15, 30 or 60 fish/200 L) and the fish were exposed to MG at 0.1 mg/L for 24 h. The results were observed that the accumulative concentration of muscular MG and LMG from the trial fish of lowest reared density (15 fish/200 L) were significant higher than those from the highest reared density. The trial fish were transferred promptly into clean water by the end of MG waterborne exposure. The correlation between reared densities and MG concentration at 6th day were insignificant.
3. This experiment focused on three different reared environments (sea, brackish or fresh-water) and the fish were exposed to MG at 0.1 mg / L. The result shown that the accumulative concentration of muscular MG and LMG of the fish from sea water were significant higher than those from brackish or fresh water. The total or any concentration of muscle MG and LMG residues from sea water group were lower than those from brackish or fresh water group, when the fish were transferred to clean water and past 6 day.
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