| Summary: | 碩士 === 長庚大學 === 生化與生醫工程研究所 === 96 === Many ingredients from plants, such as capsaicin and epigallocatechin-3-gallate (EGCG), have been reported to inhibit the proliferation of human leukemia, gastric cancers, and hepatic carcinoma cells in vitro. In this report, the inhibitive potential of capsaicin and the synergistically inhibitive effects of capsaicin and other anticancer natural compound on a hepatocellular carcinoma cell line (SK-Hep-1) were evaluated. The inhibition concentration of 50% growth (IC50) for capsaicin and EGCG were 64.4μM and 100μM, respectively. The combination of capsaicin with EGCG could inhibit further 7% to 22% of cell growth as compared with the separate treatments. The population in G1 phase and sub-G1 phase of hepatocellular carcinoma after the treatment of 60 μM and 40 μM capsaicin was increased about 16.37% and 19.07%, respectively as compared with the untreated control. From the results, capsaicin can induce hepatocellular carcinoma via the apoptosis pathway. Additionally, the inhibition of capsaicin on human primary cells (mesenchymal stem cells) was not significant. From our results, capsaicin combined with EGCG has potential to be a therapeutic candidate for hepatocellular carcinoma.
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