I.Yeast two-hybrid screening of Saccharomyces cerevisiae JHD1II. Heterologous expression and purification of nattokinase from Bacillus subtilis TKU007

碩士 === 淡江大學 === 生命科學研究所碩士班 === 96 === In part I, high efficiency transformation by electroporation is applied to yeast two¬- hybrid screening. JHD1/YER051W is a demethylase gene from Saccharomyces cerevisiae BY4742. The JHD1/YER051W ORF is ligated to the BD vector as the “bait”. Appropriate DNA libr...

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Bibliographic Details
Main Authors: Meng-Ping Lin, 林孟平
Other Authors: Ming-kai chern
Format: Others
Language:zh-TW
Published: 2008
Online Access:http://ndltd.ncl.edu.tw/handle/25390585212948136148