I.Yeast two-hybrid screening of Saccharomyces cerevisiae JHD1II. Heterologous expression and purification of nattokinase from Bacillus subtilis TKU007
碩士 === 淡江大學 === 生命科學研究所碩士班 === 96 === In part I, high efficiency transformation by electroporation is applied to yeast two¬- hybrid screening. JHD1/YER051W is a demethylase gene from Saccharomyces cerevisiae BY4742. The JHD1/YER051W ORF is ligated to the BD vector as the “bait”. Appropriate DNA libr...
Main Authors: | , |
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Other Authors: | |
Format: | Others |
Language: | zh-TW |
Published: |
2008
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Online Access: | http://ndltd.ncl.edu.tw/handle/25390585212948136148 |