Lactic Acid Bacteria ,Black Wheat Juice and Fruit Vegetable Fermentant on SD Rat Intestinal Microflora and Serum Antioxidant Enzyme Activity

• Main Authors: Chen,Ai-Hwa, 陳僾華
• Other Authors: Huang,Hui-Yu
• Format: Others
• Language: zh-TW
• Published: 2011
• Online Access: http://ndltd.ncl.edu.tw/handle/29225446005828730020

碩士 === 實踐大學 === 食品營養與保健生技學系碩士班 === 99 === This study was divided into two parts: The aim of first part study,was evaluate the effect of lactic acid bacteria and/or fruits vegetable fermentant in rat intestinal microflora and serum antioxidant capacity.Sixty Sprague-Dawley rats were randomly divided into six groups : control group, group A (lactic acid bacteria 3.60 × 107 CFU), group B (lactic acid bacteria 3.60 × 108 CFU), group C (lactic acid bacteria 7.20 × 108 CFU), group D (lactic acid bacteria 3.60 × 107 CFU, fruits and vegetables hydrolyzate 178mg/kg/day), group E (fruits and vegetables hydrolysates 178mg/kg/day), fecal samples were collected at weeks 4 and 8 for intestinal microflora determination.The serum Catalase, SOD, GSH, GPX were examinated for antioxidant capacity.The results showed that total anaerobes, bifidobacteria, lactobacilli counts increased in group A, B, C, D and E when compared with control group at the 4 week period and 8 week period in the fecal and cecum.The E.coli and Clostridium perfringens counts decreased in group A, B, C, D and E when compard with control group at the 4 week period and 8 week period in the fecal and cecum. The Catalase, SOD, GSH antioxidant capacity increased in group A, B, C, D and E when compared with the control group. GPX antioxidant capacity increased in group B, C, D and E when compared with control group. The aim of second part study, was to evaluate the effect of black wheat juice extract on rat intestinal microflora. Fifty Sprague-Dawley rats were randomly divided into five groups : control group, group A (4.12 g/kg bw /day), group B (8.23 g/kg bw /day), group C (16.26 g/ kg bw/day) and group D (41.16 g/ kg bw/day).Fecal samples were collected at weeks 2, 4 and 8 for intestinal microflora determination.The results showed that total anaerobes, bifidobacteria, lactobacilli counts increased in group A, B, C and D when compared with control group at the 2 week period, 4 week period and 8 week period in the fecal and cecum.The E.coli counts decreased in group B, C and D when compared with control group at 2 week period on the fecal, the E.coli amd Clostridium perfringens counts decreased in group A, B, C and D when compared with control group at the 4 week period and 8 week period. Therefore, lactic acid bacteria and/or fruits vegetable fermentant can increase the serum antioxidant capacity and improve the intestinal microflora.Black wheat juice also can improve the intestinal microflora.Lactic acid bacteria, fruits vegetable fermentant and black wheat juice can improve the intestinal microflora through to maintain normal bowel function.