Physiological effects of shark cartilage
碩士 === 大仁科技大學 === 食品科技研究所 === 99 === Shark cartilage (SC) is a natural food ingredient found in the ocean.Although it is considered a waste food in fish markets, it is highly nutritious.As reported, it contains mucopolysaccharides and glycosaminoglycans about 355 μg/mL and 1.17 mg/mL respectively. T...
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ndltd-TW-099TAJ052520072016-04-04T04:16:56Z http://ndltd.ncl.edu.tw/handle/96400073498625155055 Physiological effects of shark cartilage 鯊魚軟骨之生理效用 I-shan Chen 陳怡珊 碩士 大仁科技大學 食品科技研究所 99 Shark cartilage (SC) is a natural food ingredient found in the ocean.Although it is considered a waste food in fish markets, it is highly nutritious.As reported, it contains mucopolysaccharides and glycosaminoglycans about 355 μg/mL and 1.17 mg/mL respectively. The purpose of this research is to investigate the effects of shark cartilage on anti-oxidation with in vitro and ex vivo studies. The in vitro results showed that SC (i.e. 1.5, 3, 7.5, 15 and 30 mg/mL) all exhibited strong 1,1-diphenyl-2-picryl- hydrazy (DPPH) radical scavenging activities and ferrous ion chelating abilities, reaching 68~70 % and 90 % respectively and growing as the concentrations increase. In the assay of intracellular lipid per oxidation levels with NRK-52E cells, thiobarbituric acid reactive (TBARs) substances levels and glutathione (GSH) expressions were monitored for SC (i.e. 0.1, 0.5 and 1 mg/mL) co-treated with 10 mM t-BHP. The results showed that the TBARs levels were significantly reduced 48% for SC 1 mg/mL respectively, and the GSH expressions were significantly increased for all the concentrations. The study results suggested that extracts of shark cartilage have strong antioxidant activities. In addition, the IL-1β 5 ng is used to induce NRK-52E cells inflammation and SC (i.e. 0.1, 0.5 and 1 mg/mL) are added and cultured at 37°C to evaluate the anti-inflammatory ability after 24 hours. Measured analysis of the survival rate, NO and PGE2. In addition, 3 hours measured analysis of the COX-2, p-IκB and NF-κB. The results showed that SC (i.e. 0.1, 0.5 and 1 mg/mL) compared with the control group, the survival rate is no change, Reduce the generation of PGE2 and COX-2 performance (p<0.05). NRK-52E cells were phosphate into p-IκB, and inhibition of NF-κB activation. From this study showed that SC inhibited NRK-52E cells cells treated with t-BHP-induced oxidative damage and IL-1β-induced inflammation and its mechanism of action may regulate the antioxidant content and the inhibition of NF-κB DNA activation effect, and reduce inflammation of the situation. The animal experiments showed that SC (ie 8, 40, 80 and 160 mg / kg BW) were feeding for 12 weeks, not effect growth characteristics, percentage of organs weight of body weight, blood analysis in Wistar rats, compared with the control group showed, the assessment of liver and kidney function seen in rats with different concentrations of SC will not affect GPT and BUN, in the 160 mg/kg BW and the control group decreased significantly compared to GOT; In liver and kidney biopsy can see, compared with the control group showed did not significantly different. In addition, red blood cells of GSH and TBARs content of the performance, GSH showed no significant difference, but in the 160 mg/kg BW compared with the control group significantly decreased TBARs levels. Biopsy in the femur and tibia compared with the control group there was no significant different. The results showed SC could enhance the antioxidant capacity and the ability to suppress inflammation in vitro and Ex vivo. In addition, the SC might inhibit NF-κB DNA binding activation and alleviate inflammation. In animal models, SC will not result in the Wistar rat bone changes. none 王進琦 2011 學位論文 ; thesis 103 zh-TW |
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碩士 === 大仁科技大學 === 食品科技研究所 === 99 === Shark cartilage (SC) is a natural food ingredient found in the ocean.Although it is considered a waste food in fish markets, it is highly nutritious.As reported, it contains mucopolysaccharides and glycosaminoglycans about 355 μg/mL and 1.17 mg/mL respectively. The purpose of this research is to investigate the effects of shark cartilage on anti-oxidation with in vitro and ex vivo studies. The in vitro results showed that SC (i.e. 1.5, 3, 7.5, 15 and 30 mg/mL) all exhibited strong 1,1-diphenyl-2-picryl- hydrazy (DPPH) radical scavenging activities and ferrous ion chelating abilities, reaching 68~70 % and 90 % respectively and growing as the concentrations increase. In the assay of intracellular lipid per oxidation levels with NRK-52E cells, thiobarbituric acid reactive (TBARs) substances levels and glutathione (GSH) expressions were monitored for SC (i.e. 0.1, 0.5 and 1 mg/mL) co-treated
with 10 mM t-BHP. The results showed that the TBARs levels were significantly reduced 48% for SC 1 mg/mL respectively, and the GSH expressions were significantly increased for all the concentrations. The study
results suggested that extracts of shark cartilage have strong antioxidant activities. In addition, the IL-1β 5 ng is used to induce NRK-52E cells inflammation and SC (i.e. 0.1, 0.5 and 1 mg/mL) are added and cultured at 37°C to evaluate the anti-inflammatory ability after 24 hours. Measured analysis of the survival rate, NO and PGE2. In addition, 3 hours measured analysis of the COX-2, p-IκB and NF-κB. The results showed that SC (i.e. 0.1, 0.5 and 1 mg/mL) compared with the control group, the survival rate is no change, Reduce the generation of PGE2 and COX-2 performance (p<0.05). NRK-52E cells were phosphate into p-IκB, and inhibition of NF-κB activation. From this study showed that SC inhibited NRK-52E cells cells treated with t-BHP-induced oxidative damage and IL-1β-induced
inflammation and its mechanism of action may regulate the antioxidant content and the inhibition of NF-κB DNA activation effect, and reduce inflammation of the situation.
The animal experiments showed that SC (ie 8, 40, 80 and 160 mg / kg BW) were feeding for 12 weeks, not effect growth characteristics, percentage of organs weight of body weight, blood analysis in Wistar rats, compared with
the control group showed, the assessment of liver and kidney function seen in rats with different concentrations of SC will not affect GPT and BUN, in the 160 mg/kg BW and the control group decreased significantly compared to
GOT; In liver and kidney biopsy can see, compared with the control group showed did not significantly different. In addition, red blood cells of GSH and TBARs content of the performance, GSH showed no significant difference,
but in the 160 mg/kg BW compared with the control group significantly decreased TBARs levels. Biopsy in the femur and tibia compared with the control group there was no significant different.
The results showed SC could enhance the antioxidant capacity and the ability to suppress inflammation in vitro and Ex vivo. In addition, the SC might inhibit NF-κB DNA binding activation and alleviate inflammation. In animal models, SC will not result in the Wistar rat bone changes.
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author2 |
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author_facet |
none I-shan Chen 陳怡珊 |
author |
I-shan Chen 陳怡珊 |
spellingShingle |
I-shan Chen 陳怡珊 Physiological effects of shark cartilage |
author_sort |
I-shan Chen |
title |
Physiological effects of shark cartilage |
title_short |
Physiological effects of shark cartilage |
title_full |
Physiological effects of shark cartilage |
title_fullStr |
Physiological effects of shark cartilage |
title_full_unstemmed |
Physiological effects of shark cartilage |
title_sort |
physiological effects of shark cartilage |
publishDate |
2011 |
url |
http://ndltd.ncl.edu.tw/handle/96400073498625155055 |
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