Functional evaluation of lactic acid bacteria to anti-allergy in vitroand in vivo.

• Main Authors: Chen, Yu-Shin, 陳鈺馨
• Other Authors: Hsu, Chuan-Liang
• Format: Others
• Language: zh-TW
• Published: 2012
• Online Access: http://ndltd.ncl.edu.tw/handle/75575660254514065670

碩士 === 東海大學 === 食品科學系 === 100 === Allergy is regarded as body discomfort resulted from immune dysfunction. Research indicated the effect of lactic acid bacteria (LAB) on reducing allergy symptoms, as they enhanced the immune reaction of T helper 1 (Th1) and inhibited the immune reaction of T helper 2 (Th2) to reduce the allergy reaction. Three LAB strains (HK006, HK109, and HK301) were selected for testing the acid, bile salt tolerance, and absorptive capacity in this study. Furthermore, the reduced capacity of allergy immune reaction was evaluated with cells and animals. For cell tests, the three LAB strains were utilized for stimulating the macrophages in the abdominal cavity of mice RAW264.7 and human periphenal blood mononuclear cells (PBMC). Then, enzyme-linked immunosorbent assay (ELISA) was applied to analyzing the cytokine generated from the simulation of LAB, such as Interferon-γ (IFN-γ), Interleukin-12 (IL-12), Interleukin-4 (IL-4) , and Interleukin-10 (IL-10), to evaluate the function of LAB reducing allergy. For animal tests, BALB/c male mice were randomly divided into five groups which were tube fed with sterile water and fed with HK006, HK109, HK301, and the commercial product for seven weeks. In the fourth and the sixth week, the abdominal cavity was injected with Ovalbumin (OVA); and, the serum was collected from the orbital sinus in the zero, fourth, and sixth week for OVA-specific immunoglobulin E (IgE) test. After seven weeks, they were sacrificed for blood and splenocytes collection to test the OVA-specific IgE in blood and the secretions of IFN-γ, IL-12, and IL-4 in splenocytes. The results showed HK109 (206 ± 2.57 CFU / cell) presented the best absorptive capacity, both HK301 (3.88 Log CFU / mL) and HK006 (3.78 ± 0.15 Log CFU / mL) revealed the best acid tolerance, and HK301 (8.51 ± 0.12 Log CFU / mL) appeared the best bile salt resistance capacity. In regard to in-vitro tests, HK109 caused the cell strain secreting IL-12 and IFN-γ being 81 and 430 times more than the control group in the test with RAW264.7 cell strain, while the secreted IL-12 and IFN-γ resulted from the stimulation of HK301 and HK109 bacterial strains were 16 and 14 times more than the control group. With the cell tests, the three Lactobacili presented the capability of reducing allergy reactions, in which HK109 appeared the best capacity. In animal tests of the IgE concentration in the serum, HK301 and HK109 revealed the IgE descending rates about 37% and 41%, where HK109 appeared larger IgE descending in mice. In terms of splenocytes secreting cytokine, the inducing secretion of HK109, which presented 14 times for IFN-γ and 5.2 times for IL-12, was more than spontaneous. The three LAB strains could enhance the secretion of cytokine in Th1 reaction to promote the effect of immunity. To sum up the in vitro and animal test results, the three LAB strains could potentially improve allergy, in which HK109 appeared the best effect, followed by HK006 and HK301.