| Summary: | 碩士 === 大同大學 === 生物工程學系(所) === 100 === This study is to evaluate the sub-acute toxicity by administrating the extract of solid cultivation fruiting bodies (WS027), solid-state culture mycelium (GS100) or submerged culture mycelium (SB011) of Taiwanofungus camphoratus for consecutive 28 days via oral gavage in Sprague-Dawley (SD) rats.
Eighty Sprague-Dawley (SD) rats will be allocated randomly into four groups (ten males and females per group), including a control group and three treatment groups. Treatment group will be administrated at 3000 mg / kg / day of WS027, GS100 or SB011 suspension, and the control group will be administrated 0.5% CMC solution (control solution). Dosing volume was 15 mL / kg.
Experimental evaluation (DOH, 2000) included the results of the animal appearance, clinical signs, mortality, body weight, food consumption, clinical pathology (urine, blood, and serum biochemical), gross lesions, organ weight, relative organ weight and histopathological examination.
All animal survived to the end of the study. They are similar in body weight and weight growth between the treatment and control groups. No treatment-related changes in food consumption, organ weight, and related organ weight among all groups. No related changes were found in clinical pathology of all groups.
Necropsy and histopathological examination indicated no treatment-related gross lesions. Only minimal to mild hyperplasia and hypertrophy in duodenal mucosal epithelium was observed. However, the change in the duodenal mucosa epithelium is minor and won’t be considered toxic effect.. No toxic signs or treatment-related changes were observed by administrating the extract of solid cultivation fruiting bodies (WS027), solid-state culture mycelium (GS100) or submerged culture mycelium (SB011) of Taiwanofungus camphoratus for consecutive 28 days via oral gavage in Sprague-Dawley (SD) rats. Therefore, it will not cause animal toxicity by administrating15 times of recommended human daily dose (2000 mg / day).
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