Studies on Screening Culture and Dish Culture of Antrodia cinnamomea

• Main Authors: LIN, YU-TUNG, 林禹彤
• Other Authors: LIU, BING-LAN
• Format: Others
• Language: zh-TW
• Published: 2017
• Online Access: http://ndltd.ncl.edu.tw/handle/rb4hu4

碩士 === 朝陽科技大學 === 應用化學系生化科技碩博士班 === 105 === Antrodia cinnamomea, have host specificity and unique fungi in Taiwan. The separation of technology can be seen Antioxidant ingredients, including: polysaccharides, triterpenes, superoxide dismutase enzymes, adenosine, vitamins, trace elements, sterols and lignin. Many studies have confirmed that A. cinnamomea is rich in polysaccharides and triterpenoids that have medicinal effects in anti-cancer, anti-inflammation, antihypertensive and anti-oxidation. Thus, most studies were focused on polysaccharides and triterpenes. Finally, A. cinnamomea is more suitable for preservation of diced fungi. Due to the supplying limitation of camphor wood, the only source of growing fruiting body of A. cinnamomea, this research attempted to use the different medium to testing the possibility of fruiting of A. cinnamomea in a dish culture. The culture medium was prepared by using the formula medium and recycled wood flour. Selected bioactive components were analyzed by HPLC. After culture, it was found that the optimum concentration of malt extract was 4% and the optimal concentration of brown sugar was 1%. Using recycled wood flour medium by adding grapefruit powder culture had better effects on the growth and bioactive components production, The optimum concentration of grapefruit powder was 0.5%.