| Summary: | 碩士 === 國立中興大學 === 生物產業機電工程學系所 === 105 === This study examined the efficiency of enzymatic hydrolysis transformation by different particle sizes and different ratio of the corn stover powder to the citric acid liquid (solid-liquid ratio). There are four particle mean sizes: 0.20824mm, 0.12364mm, 0.08772mm, and 0.05232mm; and two solid-liquid ratio: 1:8 and 1:10. The process of enzymatic hydrolysis and saccharification was processed in the condition of ph4.3-4.5, temperature of 50-52℃ and countinuous stirring. The particle mean size of 0.05232mm had the better result.The saccharification transformation is up to 75% and the final concentration is the highest. Moreover, the sugar concentration during saccharification in first hour increased significantly. In the first hour, the study observed the sugar concentration increasing about 0.12% every 30 seconds in the first 5 minutes, and after 5 minutes, it increased 0.11% every 5 minutes untill the first hour. Furthermore, the minimum experimental time is only 6 hours for the particle mean sizes larger than 0.149mm and 26 hours for the sizes smaller than 0.149mm. For the different solid-liquid ratio, the result showed the sugar conversion rate of the solid-liquid ratio 1:10 is higher than the ratio 1:8, although the final sugar concentration of the solid-liquid ratio 1:8 is higher than the ratio 1:10. In other words, the solid-liquid ratio 1:10 transformed cellulose and hemicellulose to sugar more efficiently. Above all, this study has simulated the enzymatic hydrolysis transformation of corn stover for particle mean size 0.149mm and 0.05232mm, and the R2 are 0.93 and 0.896 respectively. Both of the simulated equations are highly correlation. The simulated mathematics model can be applied to describe enzymatic hydrolysis transformation.
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