| Summary: | 碩士 === 國立臺灣師範大學 === 化學系 === 106 === Due to the increase of popularity and the change of global climate environment, the concern on global food crisis is growing. This phenomenon has caused the genetically modified organisms (GMOs) thriving in recent years. Although the GMOs can dramatically solve the global food crisis, there are some unknown anxieties and the safety issues which are suspicious to the public now. In this study, isobaric tags for relative and absolute quantitation (iTRAQ) technology was applied for the investigation of the protein profiles in both the genetically modified (GM, DAS-81419-2) and the non-genetically modified (non-GM) soybeans. The iTRAQ labeled peptides were fractionated by solution isoelectric focusing (sIEF), strong cationic exchange chromatography (SCX) and basic reverse phase chromatography (bRP), followed by nano-LC tandem mass spectrometric analysis. Two-dimensional liquid chromatography technique that employed on iTRAQ labeled peptides gave results with excellent complementarity, orthogonality and more protein identifications. A total of 2648 proteins including 8831 unique peptides were identified. Moreover, differentially expressed proteins were selected between the GM and the non-GM soybeans for the bioinformatics analysis. They were found in ribosome, nutrient reservoir activity, cytoplasmic pathway, and so on. We expect that it can be beneficial on its future development for genetically modified crops.
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