Effects of culture conditions on production and composition of polysaccharides and ganoderic acids of Ganoderma formosanum and Ganoderma lucidum

• Main Authors: Hsin-Hua Lee, 李幸樺
• Other Authors: 呂廷璋
• Format: Others
• Language: zh-TW
• Published: 2018
• Online Access: http://ndltd.ncl.edu.tw/handle/mey4cw

碩士 === 國立臺灣大學 === 食品科技研究所 === 106 === Ganoderma formosanum and Ganoderma lucidium, Lingzhi, are traditional Chinese medicine and are considered as healthy food. The bioactive components in Lingzhi are (1, 3；1, 6)-β-D-glucans and ganoderic acids. This study aims to investigate that the effect of the static culture, the submerged culture and the two-stage fermentation on the production of the (1, 3；1, 6)-β-D-glucans and ganoderic acids of Ganoderma formosanum and Ganoderma lucidum. The biomass production, polysaccharide in mycelium and broth, the content and the degree of the branch of (1,3；1,6)-β-D-glucans and the structure of ganoderic acids of Ganoderma formosanum and Ganoderma lucidum were compared. In this study, the (1,3)-β-D-glucanase was used to investigate the content and the degree of the branch of (1,3；1,6)-β-D-glucans. Liquid chromatography-tandem mass spectrometry was used to analyze ganoderic acids. The structure properties of ganoderic acids were identified by accurate m/z (-2.33-2.33 ppm), MS/MS spectra and UV spectra. Standard addition method was used to analyze the concentration of ganoderic acids. Relative quantification method was used to analyze the ganoderic acids without an available commercial standard. In this research, the result shows that static culture produces more ganoderic acid than submerge culture. However, shaking culture produces more biomass and polysaccharide. Two-stage fermentation can enhance the yield of the target compound. The mycelium of Ganoderma formosanum has maximum biomass by static culture. At 10 days, Ganoderma formosanum has the maximum yield of polysaccharides in the mycelium and the broth. By shaking culture, the maximum biomass of the mycelium of Ganoderma lucidum is at 15 days and the maximum yield of the polysaccharide of Ganoderma lucidum is at 10 days but there is no significant difference in the broth. At 30 days, by static culture, the content of (1,3；1,6)-β-D-glucan of Ganoderma formosanum mycelia is more than Ganoderma lucidum. The degree of the branch of (1,3；1,6)-β-D-glucan of Ganoderma formosanum mycelia is between 0.2-0.33. Ganoderma lucidum only produces (1,3；1,6)-β-D-glucan in static culture and the degree of the branch of (1,3；1,6)-β-D-glucan is lower than 0.2-0.33. Ganoderma lucidum contains more mycelia and polysaccharides than Ganoderma formosanum. However, the percentage of (1,3；1,6)-β-D-glucan in the polysaccharides of Ganoderma formosanum is more than Ganoderma lucidum. Ganoderma lucidum and Ganoderma tsugae fruiting body were used as QC sample. 27 kinds of ganoderic acids were found in QC sample. In this study, the mycelium of Ganoderma formosanum in static culture 30 days has more ganoderic acid H than Ganoderma lucidum. The static culture and shaking culture have no significant effect on the production of Ganoderic acid H of Ganoderma lucidum.