Antioxidant and Anti-Senescence Effects of Chinese Herbal Medicines and Fermentation Products of Bacillus subtilis

• Main Authors: Chen-Yu Huang, 黃晨瑀
• Other Authors: 劉嚞睿
• Format: Others
• Language: zh-TW
• Published: 2018
• Online Access: http://ndltd.ncl.edu.tw/handle/mu6kcu

碩士 === 國立臺灣大學 === 動物科學技術學研究所 === 106 === Chinese herbal medicines (CHMs), which possess immunomodulatory, anti-oxidant, and anti-inflammatory activities, have been used as traditional medicines for thousands of years. Fermentation is a traditional technology for improving the efficacy or reducing the adverse effects of CHMs. The objective of this study is to investigate the antioxidant and anti-senescence activities of the CHMs, including Gardenia jasminoides, Scutellaria baicalensis, Coptidis rhizome, Glycyrrhizae radix et rhizome, Chrysanthemi flos, Bupleuri radix, and Baphicacanthi cusiae Rhizoma et radix. The antioxidant and anti-senescence activities of the CHMs fermented by Bacillus subtilis were also studied. The CHMs were inoculated with B. subtilis AC and were incubated at 37°C for 24 h. The active ingredients of the CHMs before and after fermentation were analyzed by using high performance liquid chromatography (HPLC). The antioxidant activities of the CHMs and their fermentation products were measured by DPPH radical photometric assay and deoxyribose assay. For the establishment of premature senescence cellular model, human lung fibroblast MRC-5 cells were treated with H2O2, and the cell cycle was analyzed by flow cytometry. The MRC-5 cell before hydrogen peroxide (H2O2)-induced oxidative damage were pretreated with CHMs or the fermentation products of CHMs, and then the major indexes of oxidative damage, including superoxide dismutase (SOD) and glutathione peroxidase (GPx), were quantified by biochemical assays. The results indicated that the DPPH scavenging activities of Gardenia jasminoides, Scutellaria baicalensis, Coptidis rhizome, Glycyrrhizae radix et rhizome, Chrysanthemi flos, Bupleuri radix, and Baphicacanthi cusiae Rhizoma et radix were elevated by fermentation. For deoxyribose assay, only Gardenia jasminoides, Scutellaria baicalensis, and Glycyrrhizae radix et rhizome showed significantly difference after fermentation (p<0.05). The active ingredients of Scutellaria baicalensis, Glycyrrhizae radix et rhizome, and Bupleuri radix were elevated by fermentation (p<0.05). On the other hand, the results of cell cycle assay showed that H2O2-induced oxidative damage, leading to G2 arrest of the cell cycle. Gardenia jasminoides and Glycyrrhizae radix et rhizome have best capacity to relieve G2 arrest before fermentation, while Scutellaria baicalensis, Chrysanthemi flos, Bupleuri radix, and Baphicacanthi cusiae Rhizoma et radix have best capacity after fermentation. Furthermore, the results of SOD activity assay showed that Baphicacanthi cusiae Rhizoma et radix has best activity before fermentation, however, Baphicacanthi cusiae Rhizoma et radix has the lowest activity after fermentation. Moreover, the results of GPx activity showed that Scutellaria baicalensis has best activity before fermentation, while Chrysanthemi flos and Bupleuri radix have best activity after fermentation. To sum up, the CHMs may contain a wide variety of free radical scavenging molecules, which are rich in antioxidant activity. The medicinal value can be enhanced by microbial fermentation. Last but not least, we are looking forward to conducting animal experiment and developing health food.