| Summary: | 碩士 === 國立中興大學 === 化學系所 === 107 === Food safety becomes important issue of human health in last years. The trace mycotoxins in food cause the damage of human health, hence, to develop a rapid, sensitive for analysis trace mycotoxins in food is very important for food safety. This study developed the dispersive liquid-liquid microextraction (DLLME) combined by LC-MS/MS for determine aflatoxin B1 (AFB1), aflatoxin B2 (AFB2), aflatoxin G1 (AFG1), aflatoxin G2 (AFG2), and ochratoxin A (OTA) in cereal products. The parameter of extraction including selection of extraction and disperser solvent and its volume, influence of pH and salt were systematically evaluated. The ionization of was carried out by ESI and the selected ion monitoring (MRM) mode was used as scan mode for five mycotoxins. The protonated molecules ([M+H]+) of AFB1 (m/z 313), AFB2 (m/z 315), AFG1 (m/z 329), and AFG2 (m/z 331) were obtained as base peaks by using positive ionization mode. The deprotonated molecules ([M–H]–) of OTA (m/z 402) was obtained as base peak by using negative ionization mode. The correlation coefficient. The correlation coefficient (R2) above 0.9969. The limit of detection and quantification of each analytes obtained 201.11 µgL-1 and 670.38 µgL-1 (aflatoxin B1), 158.17 µgL-1 and 527.22 µgL-1 (aflatoxin B2), 811.56 µgL-1 and 404.43 µgL-1 (aflatoxin G1), 600.86 µgL-1 and 468.99 µgL-1 (aflatoxin G2) and 54.37 µgL-1 and 194.99 µgL-1 (ochratoxin A). The DLLME method can save time, consumption of low solvents, simple and fast technique and obtain high extraction efficiency.
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