| Summary: | 碩士 === 國立中興大學 === 食品安全研究所 === 107 === Allergy is a reaction that immune system has hypersensitivity when host cell exposed to external allergen including pollen, food, dust mites, cosmetics, mold spores and the others. Common allergic diseases such as food allergies, asthma, allergic rhinitis, urticaria and dermatitis are type I allergic reactions.
In our previous study, soluble oxalate, the main active compound of 95% ethanol extractsn of Colocasia esculenta var. antiquorum corm, could inhibit host cell inflammation, which probably due to its ability to chelate calcium ions stimulated by H. pylori. Therefore, in this study, the inhibitions of β-hexosaminidase release and cytosolic Ca2+ concentrations of the eighteen 95% ethanol extracts of natural products with different oxalate contents were investigated. In the meantime, chemical compositions of the eighteen extracts were analyzed, including soluble oxalate, total oxalate, total flavonoids, total phenols and total carotenoids. After that, correlations between inhibitions of β-hexosaminidase release and chemical compositions of eighteen extracts were investigated using spearman’s correlation coefficient.
The results showed that all the extracts could significantly inhibit the release of β-hexosaminidase (inhibition rate: 14.65 ± 3.31 ~ 100.55 ± 0.53%) and cytosolic Ca2+ concentrations (inhibition rate: 25.68 ± 7.89 ~ 96.34 ± 17.80%) in the IgE sensitized and DNP-HSA stimulated RBL-2H3 cells. The correlations between the inhibitions of β-hexosaminidase release and the chemical compositions (soluble oxalate, total flavonoids and total phenols) possessed high positive correlation. Therefore, the relevant mechanisms of 95% ethanol extracts of eighteen natural products might be that soluble oxalate in the extracts chelated calcium ions and then reduced intracellular Ca2+ concentration, eventually, inhibited the release of β-hexosaminidase. On the other hand, the flavonoids and the phenolic acid compounds in eighteen extracts might inhibit protein (LAT, PLCγ, IP3, PKC, etc.) expressed which regulated β-hexosaminidase release in IgE sensitized and DNP-HSA stimulated RBL-2H3 cells.
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