Rapid Detection Method Of Histamine With Surface Enhanced Raman Spectroscopy In Fishes

碩士 === 元培醫事科技大學 === 食品科學系碩士班 === 107 === Histamine poisoning is one of the often occurred food poisoning incidents, due to the histidine was transformed into histamine by bacteria under an undesirable storage conditions. Therefore, the examination of sea food freshness is an important indicator to p...

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Main Authors: PAI,YU-CHEN, 白羽宸
Other Authors: LIN,HSI-PIN
Format: Others
Language:zh-TW
Published: 2019
Online Access:http://ndltd.ncl.edu.tw/handle/c26a64
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spelling ndltd-TW-107YUST02530012019-05-30T03:57:15Z http://ndltd.ncl.edu.tw/handle/c26a64 Rapid Detection Method Of Histamine With Surface Enhanced Raman Spectroscopy In Fishes 表面增強拉曼光譜在魚肉中組織胺快速檢測方法與應用 PAI,YU-CHEN 白羽宸 碩士 元培醫事科技大學 食品科學系碩士班 107 Histamine poisoning is one of the often occurred food poisoning incidents, due to the histidine was transformed into histamine by bacteria under an undesirable storage conditions. Therefore, the examination of sea food freshness is an important indicator to prevent food poisoning. Thus, the development of rapid and accurate methods for histamine is very important to ensure the sea food quality. In this study, we used the surface enhanced Raman spectroscopy to develop a new detection method of histamine. No signal appeared directly with histamine standards. It was also suggested a linear relationship of the calibration curve (R2=0.92) after incubated with histamine dehydrogenase with a specificity for histamine. But it did not appear any significant signal when determined with histamine standards were added into fish extract and incubated with histamine dehydrogenase. However, it appeared some obvious signals after adding 0.75 M potassium chloride (KCl) and has a good linear relationship of the calibration curve and the regression coefficient (R2) can reach above 0.97, but the maximum concentration of determination only reached 30 ppm. Then we used Dansyl chloride (5-(dimethylamino) naphthalene-1- sulfonyl chloride) to carry out a derivatizing reaction with histamine standard and to determine after adding saturated sodium chloride solution. The result shows some obvious specific signals and 1273 cm-1 Raman Shift has a very good Linear relationship of the calibration curve (R2>0.99), the maximum concentration of determination also up to 50 ppm. But it was unable to determine because of the interference from background noises when histamine standard was added into fish extract extracted and derivatized as above method. Therefore, after removing the background value to observe the clear signal, this method will be a fast and effective histamine detection method in the future. LIN,HSI-PIN LIN,CHUNG-SAINT 林錫斌 林仲聖 2019 學位論文 ; thesis 67 zh-TW
collection NDLTD
language zh-TW
format Others
sources NDLTD
description 碩士 === 元培醫事科技大學 === 食品科學系碩士班 === 107 === Histamine poisoning is one of the often occurred food poisoning incidents, due to the histidine was transformed into histamine by bacteria under an undesirable storage conditions. Therefore, the examination of sea food freshness is an important indicator to prevent food poisoning. Thus, the development of rapid and accurate methods for histamine is very important to ensure the sea food quality. In this study, we used the surface enhanced Raman spectroscopy to develop a new detection method of histamine. No signal appeared directly with histamine standards. It was also suggested a linear relationship of the calibration curve (R2=0.92) after incubated with histamine dehydrogenase with a specificity for histamine. But it did not appear any significant signal when determined with histamine standards were added into fish extract and incubated with histamine dehydrogenase. However, it appeared some obvious signals after adding 0.75 M potassium chloride (KCl) and has a good linear relationship of the calibration curve and the regression coefficient (R2) can reach above 0.97, but the maximum concentration of determination only reached 30 ppm. Then we used Dansyl chloride (5-(dimethylamino) naphthalene-1- sulfonyl chloride) to carry out a derivatizing reaction with histamine standard and to determine after adding saturated sodium chloride solution. The result shows some obvious specific signals and 1273 cm-1 Raman Shift has a very good Linear relationship of the calibration curve (R2>0.99), the maximum concentration of determination also up to 50 ppm. But it was unable to determine because of the interference from background noises when histamine standard was added into fish extract extracted and derivatized as above method. Therefore, after removing the background value to observe the clear signal, this method will be a fast and effective histamine detection method in the future.
author2 LIN,HSI-PIN
author_facet LIN,HSI-PIN
PAI,YU-CHEN
白羽宸
author PAI,YU-CHEN
白羽宸
spellingShingle PAI,YU-CHEN
白羽宸
Rapid Detection Method Of Histamine With Surface Enhanced Raman Spectroscopy In Fishes
author_sort PAI,YU-CHEN
title Rapid Detection Method Of Histamine With Surface Enhanced Raman Spectroscopy In Fishes
title_short Rapid Detection Method Of Histamine With Surface Enhanced Raman Spectroscopy In Fishes
title_full Rapid Detection Method Of Histamine With Surface Enhanced Raman Spectroscopy In Fishes
title_fullStr Rapid Detection Method Of Histamine With Surface Enhanced Raman Spectroscopy In Fishes
title_full_unstemmed Rapid Detection Method Of Histamine With Surface Enhanced Raman Spectroscopy In Fishes
title_sort rapid detection method of histamine with surface enhanced raman spectroscopy in fishes
publishDate 2019
url http://ndltd.ncl.edu.tw/handle/c26a64
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