Expression of human cystatin c by the methylotrophic yeast pichia pastoris

Human cystatin C is a cysteine-proteinase inhibitor with great potential as a therapeutic protein. The methylotrophic yeast, Pichia pastoris, was selected as a host to express a human cystatin C variant in which site directed mutagenesis was used to introduce two consensus sequences for N-linked...

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Bibliographic Details
Main Author: Files, David Andrew
Format: Others
Language:English
Published: 2009
Online Access:http://hdl.handle.net/2429/10460