Characterization of the Pseudomonas aeruginosa penicillin-binding proteins 3 and 3x : gene cloning, expression and role in susceptibility to [beta]-lactam antibiotics
Two degenerate oligonucleotides primers were synthesized based on the amino acid sequences found in the conserved SXXK and KTG motifs of Escherichia coli high-molecular-weight PBPs and Nesseria gonorrhoeae PBP2. The primers were subsequently used in a PCR amplification experiment using Pseudomona...
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Format: | Others |
Language: | English |
Published: |
2009
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Online Access: | http://hdl.handle.net/2429/6669 |