Characterization of the Pseudomonas aeruginosa penicillin-binding proteins 3 and 3x : gene cloning, expression and role in susceptibility to [beta]-lactam antibiotics

Two degenerate oligonucleotides primers were synthesized based on the amino acid sequences found in the conserved SXXK and KTG motifs of Escherichia coli high-molecular-weight PBPs and Nesseria gonorrhoeae PBP2. The primers were subsequently used in a PCR amplification experiment using Pseudomona...

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Bibliographic Details
Main Author: Liao, Xiaowen
Format: Others
Language:English
Published: 2009
Online Access:http://hdl.handle.net/2429/6669