Characterization of DNA structural elements and conformational changes accompanying the binding of T7 RNA polymerase to its promoter
The first step of transcription initiation, promoter binding, is characterized in the T7 RNA polymerase model system. A previous two-domain model for the T7 RNA polymerase promoter is tested by the use of different fluorescence methods. The increase in the fluorescence anisotropy of a rhodamine dye...
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| Language: | ENG |
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ScholarWorks@UMass Amherst
1999
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| Online Access: | https://scholarworks.umass.edu/dissertations/AAI9920662 |