Advancements in Firefly Luciferase-Based Assays and Pyrosequencing Technology

Advancements in Firefly Luciferase-Based Assays and Pyrosequencing Technology

Pyrosequencing is a new DNA sequencing method relying on thesequencing-by-synthesis principle and bioluminometric detectionof nucleotide incorporation events. The objective of thisthesis was improvement of the Pyrosequencing method byincreasing the thermal stability of firefly luciferase, and byintr...

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Bibliographic Details
Main Author: Eriksson, Jonas
Format: Doctoral Thesis
Language:English
Published: KTH, Bioteknologi 2004
Subjects:
bioluminescence
osmolytes
glycine betaine
thermostability
firefly luciferase
inorganic pyrophosphatase
inorganic pyrophosphate
Pyrosequencing technology
secondary DNA-structures
Sequenase
Klenow-polymerase
reaction rates
temperature
Online Access:http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-3708
http://nbn-resolving.de/urn:isbn:91-7283-618-0

Internet

http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-3708
http://nbn-resolving.de/urn:isbn:91-7283-618-0

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