Development of a CRISPR systemfor gene knockouts in Diplomonad parasites

This thesis describes the attempt to implement the CRISPR-Cas9 system in the two diplomonad pathogens: Giardia intestinalis and Spironucleus salmonicida. Two approaches of the implementation were done: first the Cas9 was expressed, followed by expression of the guide RNA. Additionally, to extend the...

Full description

Bibliographic Details
Main Author: Törnblom, Viktor
Format: Others
Language:English
Published: Uppsala universitet, Institutionen för biologisk grundutbildning 2016
Online Access:http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-294365