Selection of a calcium-dependent IgG1-binding protein domain

Selection of a calcium-dependent IgG1-binding protein domain

Standard purification processes in large scale antibody production are largely dependent on Protein A chromatography. Protein A binds specifically to many subclasses of IgG with high affinity. However, in order to elute the proteins, the pH needs to be lowered. Since lowering of the pH can be detrim...

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Bibliographic Details
Main Author: Rönning, Sanne
Format: Others
Language:English
Published: Uppsala universitet, Institutionen för biologisk grundutbildning 2020
Subjects:
protein science
phage display
selection
Protein A
chromatography
calcium dependence
characterization
proteinteknik
fagdisplay
selektion
kromatografi
calciumberoende
karakterisering
Biochemistry and Molecular Biology
Biokemi och molekylärbiologi
Online Access:http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-417065

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http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-417065

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