Integrin alphα5/fibronectin1 and focal adhesion kinase are required for lens fiber morphogenesis in zebrafish
Fibronectin (fn) and integrin α5 (itgα5) are both key players in cell adhesion and intracellar signaling, however the specific in vivo role of these proteins has never been analyzed in the vertebrate lens. The results presented here indicate that Fn1 and Itgα5 proteins are essential for the proper...
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ndltd-UTEXAS-oai-repositories.lib.utexas.edu-2152-ETD-UT-2010-08-19662015-09-20T16:56:26ZIntegrin alphα5/fibronectin1 and focal adhesion kinase are required for lens fiber morphogenesis in zebrafishHayes, Julie MarieZebrafishLensIntegrinFibronectionFAKFibronectin (fn) and integrin α5 (itgα5) are both key players in cell adhesion and intracellar signaling, however the specific in vivo role of these proteins has never been analyzed in the vertebrate lens. The results presented here indicate that Fn1 and Itgα5 proteins are essential for the proper development of the lens. The loss of Fn1 protein in the zebrafish embryo results in distinct adhesion defects, defects in lens fiber morphogenesis, and cataracts. These results were phenocopied in zebrafish itga5 mutants, thereby indicating an essential role for Fn1 and Itgα5 during lens development. Furthermore, embryos with reduced levels of ptk2.1 (focal adhesion kinase – FAK) also phenocopied the defective fn1 and itgα5 lens, suggesting that FAK is a major player in the intracellular signaling mediated by Fn1/Itgα5 interactions in the lens.text2010-12-17T20:58:50Z2010-12-17T20:58:58Z2010-12-17T20:58:50Z2010-12-17T20:58:58Z2010-082010-12-17August 20102010-12-17T20:58:58Zthesisapplication/pdfhttp://hdl.handle.net/2152/ETD-UT-2010-08-1966eng |
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Others
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Zebrafish Lens Integrin Fibronection FAK |
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Zebrafish Lens Integrin Fibronection FAK Hayes, Julie Marie Integrin alphα5/fibronectin1 and focal adhesion kinase are required for lens fiber morphogenesis in zebrafish |
description |
Fibronectin (fn) and integrin α5 (itgα5) are both key players in cell adhesion and intracellar signaling, however the specific in vivo role of these proteins has never been analyzed in the vertebrate lens.
The results presented here indicate that Fn1 and Itgα5 proteins are essential for the proper development of the lens. The loss of Fn1 protein in the zebrafish embryo results in distinct adhesion defects, defects in lens fiber morphogenesis, and cataracts. These results were phenocopied in zebrafish itga5 mutants, thereby indicating an essential role for Fn1 and Itgα5 during lens development. Furthermore, embryos with reduced levels of ptk2.1 (focal adhesion kinase – FAK) also phenocopied the defective fn1 and itgα5 lens, suggesting that FAK is a major player in the intracellular signaling mediated by Fn1/Itgα5 interactions in the lens. === text |
author |
Hayes, Julie Marie |
author_facet |
Hayes, Julie Marie |
author_sort |
Hayes, Julie Marie |
title |
Integrin alphα5/fibronectin1 and focal adhesion kinase are required for lens fiber morphogenesis in zebrafish |
title_short |
Integrin alphα5/fibronectin1 and focal adhesion kinase are required for lens fiber morphogenesis in zebrafish |
title_full |
Integrin alphα5/fibronectin1 and focal adhesion kinase are required for lens fiber morphogenesis in zebrafish |
title_fullStr |
Integrin alphα5/fibronectin1 and focal adhesion kinase are required for lens fiber morphogenesis in zebrafish |
title_full_unstemmed |
Integrin alphα5/fibronectin1 and focal adhesion kinase are required for lens fiber morphogenesis in zebrafish |
title_sort |
integrin alphα5/fibronectin1 and focal adhesion kinase are required for lens fiber morphogenesis in zebrafish |
publishDate |
2010 |
url |
http://hdl.handle.net/2152/ETD-UT-2010-08-1966 |
work_keys_str_mv |
AT hayesjuliemarie integrinalpha5fibronectin1andfocaladhesionkinasearerequiredforlensfibermorphogenesisinzebrafish |
_version_ |
1716821229119209472 |