Characterization of HIV-1 Reverse Transcriptase substrate specificity by conformationally sensitive fluorescence

Characterization of HIV-1 Reverse Transcriptase substrate specificity by conformationally sensitive fluorescence

We have engineered a mutant of HIV Reverse Transcriptase that can be fluorescently labeled by covalent attachment of the environmentally sensitive fluorophore 7-diethylamino-3-((((2-maleimidyl)ethyl)amino)carbonyl)coumarin (MDCC). The result is a polymerase that is kinetically indistinguishable fr...

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Bibliographic Details
Main Author: Kellinger, Matthew William
Format: Others
Language:English
Published: 2012
Subjects:
HIV
Reverse Transcriptase
Induced fit
Drug resistance
Kinetics
Single molecule
Online Access:http://hdl.handle.net/2152/ETD-UT-2010-12-2154

Internet

http://hdl.handle.net/2152/ETD-UT-2010-12-2154

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