Preparation of brush border and basolateral membrane vesicles from bovine intestine for nutrient uptake studies
Brush border and basolateral membrane vesicles were isolated by subjecting homogenized mucosal cells from bovine small intestine to a divalent cation aggregation followed by a series of differential and density gradient centrifugations. Membrane marker enzyme assays were used to monitor the effectiv...
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| Format: | Others |
| Language: | en |
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Virginia Polytechnic Institute and State University
2020
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| Online Access: | http://hdl.handle.net/10919/101336 |