SgrAI Clustering on DNA

SgrAI restriction enzymes bind to DNA at multiple sites and move to a central location on the molecule, forming a cluster. We qualitatively confirmed this behavior using a tethered particle motion assay and a DNA stretching assay using an optical trap. Quantitative analysis proved impossible due to...

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Bibliographic Details
Main Author: Hendel, Edward Stephen
Other Authors: Visscher, Koen
Language:en
Published: The University of Arizona. 2013
Online Access:http://hdl.handle.net/10150/297647