SgrAI Clustering on DNA
SgrAI restriction enzymes bind to DNA at multiple sites and move to a central location on the molecule, forming a cluster. We qualitatively confirmed this behavior using a tethered particle motion assay and a DNA stretching assay using an optical trap. Quantitative analysis proved impossible due to...
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| Language: | en |
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The University of Arizona.
2013
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| Online Access: | http://hdl.handle.net/10150/297647 |
| Summary: | SgrAI restriction enzymes bind to DNA at multiple sites and move to a central location on the molecule, forming a cluster. We qualitatively confirmed this behavior using a tethered particle motion assay and a DNA stretching assay using an optical trap. Quantitative analysis proved impossible due to the length of our DNA molecules and the large number of SgrAI binding sites. We recommend using a plasmid with a small number of binding sites for future experiments. |
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