Design and Applications of Split-Luciferase Systems in Vitro and in Cellulo
Split-protein reassembly methods allow for the detection of a variety of macromolecular interactions in vitro, in cellulo and in vivo. Spilt-protein reporters, such as split-firefly luciferase (Fluc), depend upon the conditional reassembly of the genetically fragmented enzyme. The two fragments of F...
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| Language: | en_US |
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The University of Arizona.
2014
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| Online Access: | http://hdl.handle.net/10150/333354 |