Identification of G-quadruplexes in long functional RNAs using 7-deazaguanine RNA
RNA G-quadruplex (G4) structures are thought to affect biological processes, including translation and pre-mRNA splicing, but it is not possible at present to demonstrate that they form naturally at specific sequences in long functional RNA molecules. We developed a new strategy, footprinting of lon...
Main Authors: | , , , , , , |
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Other Authors: | |
Language: | en |
Published: |
NATURE PUBLISHING GROUP
2016
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Online Access: | http://hdl.handle.net/10150/623077 http://arizona.openrepository.com/arizona/handle/10150/623077 |
Summary: | RNA G-quadruplex (G4) structures are thought to affect biological processes, including translation and pre-mRNA splicing, but it is not possible at present to demonstrate that they form naturally at specific sequences in long functional RNA molecules. We developed a new strategy, footprinting of long 7-deazaguanine-substituted RNAs (FOLDeR), that allows the formation of G4s to be confirmed in long RNAs and under functional conditions. |
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