A regeneration study with embryonic tissues and embryonic cells of carrot, Daucus carota L. and apple, Pyrus malus L

A method for isolating embryonic cells in order to establish primary cell cultures has been developed. Embryos, intact or cut into small pieces, were digested with different cell-separating enzymes and after 24h post-digestion in 0.6M mannitol they were sheared by using a hypodermic syringe. The res...

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Bibliographic Details
Main Author: Rubos, Athanese C.
Published: University of Bath 1983
Subjects:
Online Access:https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.347036