A regeneration study with embryonic tissues and embryonic cells of carrot, Daucus carota L. and apple, Pyrus malus L
A method for isolating embryonic cells in order to establish primary cell cultures has been developed. Embryos, intact or cut into small pieces, were digested with different cell-separating enzymes and after 24h post-digestion in 0.6M mannitol they were sheared by using a hypodermic syringe. The res...
Main Author: | Rubos, Athanese C. |
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Published: |
University of Bath
1983
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Subjects: | |
Online Access: | https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.347036 |
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