Characterisation of the effect of process factors upon protein refolding yield

Expression of recombinant protein in bacteria such as Escherichia coli can result in the formation of inclusion bodies. Active protein is derived from inclusion bodies by protein refolding. Refolding yields are often poor, and are a bottleneck in such recombinant protein processes. Work has addresse...

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Bibliographic Details
Main Author: Mannall, Gareth James
Published: University College London (University of London) 2006
Subjects:
Online Access:http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.445308