Engineering archaeal DNA polymerases for biotechnology applications
The DNA polymerase from the archaeon Pyrococcus furiosus (Pfu- Pol) is commonly used in the polymerase chain reaction (PCR). The enzyme has high thermostability and is very accurate due to the presence of 3'---5'exonuclease (proofreading) activity. Unfortunately, the polymerase has relativ...
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University of Newcastle Upon Tyne
2012
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| Online Access: | http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.606814 |