Single-shot optical sectioning using polarised illumination coded structured illumination microscopy (picoSIM)

The conventional epi-uorescent wide-eld microscope features a uniform illumination of an extended sample region. A problem arises with this setup since light from outof-focus uorophores is also detected. The result is poor quality in the nal image, as out-of-focus structures appear blurred; furtherm...

Full description

Bibliographic Details
Main Author: Appelt, Daniel
Published: King's College London (University of London) 2013
Subjects:
Online Access:http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.628263