Fluorescence lifetime imaging and spectroscopy of GFP mutants, and the characterisation of FRET pairs, using high sensitivity, time- and space- correlated single photon counting
Fluorescens Lifetime Imaging Microscopy (FLIM) and fluorescence lifetime spectroscopy have been used to characterise the photophysics of different mutants of Green Fluorescent Protein (GFP) and to show evidence of Förster Resonance Energy Transfer (FRET) between different mutants in both model syste...
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University of Edinburgh
2007
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| Online Access: | http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.657807 |