The kinetic characterisation of Rhodotorula graminis L-mandelate dehydrogenase
The recombinant form of the <I>R. graminis </I><SUB>L</SUB>-mdh has been overexpressed in <I>E. coli</I> to levels around 14% of the total cell protein. The enzyme was purified to homogeneity after several ion-exchange and gel filtration columns. The steady state...
Main Author: | |
---|---|
Published: |
University of Edinburgh
1998
|
Subjects: | |
Online Access: | http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.661967 |