The kinetic characterisation of Rhodotorula graminis L-mandelate dehydrogenase

The recombinant form of the <I>R. graminis </I><SUB>L</SUB>-mdh has been overexpressed in <I>E. coli</I> to levels around 14% of the total cell protein. The enzyme was purified to homogeneity after several ion-exchange and gel filtration columns. The steady state...

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Bibliographic Details
Main Author: Sinclair, Rhona
Published: University of Edinburgh 1998
Subjects:
579
Online Access:http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.661967