Specific detection of 14-3-3 proteins by ssDNA aptamers
A library of ssDNA aptamers has been screened and a number of molecules which show high binding affinity to 14-3-3 proteins have been isolated. The gene encoding mammalian 14-3-3 γ modified by (his)<sub>6</sub> tagging, was overexpressed in <i>E. coli </i>and purified to homo...
| Main Author: | |
|---|---|
| Published: |
University of Edinburgh
2006
|
| Subjects: | |
| Online Access: | http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.662436 |