The sequence specific DNA binding activity of pogo transposase
In this thesis, the two ORFs believed to code for <I>pogo</I> transposase were joined in frame by PCR and cloned into a pGEX vector. The <I>pogo</I> transposase was fused to the C-termini of glutathione-S-transferase (GST) and expressed in <I>E. coli</I>. The DNA...
Main Author: | |
---|---|
Published: |
University of Edinburgh
1997
|
Subjects: | |
Online Access: | http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.663449 |