The sequence specific DNA binding activity of pogo transposase

In this thesis, the two ORFs believed to code for <I>pogo</I> transposase were joined in frame by PCR and cloned into a pGEX vector. The <I>pogo</I> transposase was fused to the C-termini of glutathione-S-transferase (GST) and expressed in <I>E. coli</I>. The DNA...

Full description

Bibliographic Details
Main Author: Wang, Hongmei
Published: University of Edinburgh 1997
Subjects:
Online Access:http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.663449