Design and Synthesis of 3'-Oxygen-Modified Cleavable Nucleotide Reversible Terminators for Scarless DNA Sequencing by Synthesis
This dissertation describes the design and synthesis of novel cleavable fluorescent/anchor modified nucleotide reversible terminators using 3’-O-dithiomethyl (3’-O-DTM; 3’-O-SS) as a linker to directly or indirectly attach a fluorescent reporter to achieve scarless DNA Sequencing by Synthesis (SBS)....
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| Language: | English |
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2018
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| Online Access: | https://doi.org/10.7916/D8XD2HST |