Studying and Improving Lambda Red Recombination for Genome Engineering in Escherichia coli

The phage-derived Lambda Red recombination system utilizes exogenous DNA in order to generate precise insertion, deletion, and point mutations in Escherichia coli and other bacteria. Due to its convenience, it is a frequently-used tool in genetics and molecular biology, as well as in larger-scale g...

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Bibliographic Details
Main Author: Mosberg, Joshua Adam Weintrob
Other Authors: Church, George McDonald
Language:en_US
Published: Harvard University 2013
Subjects:
Red
Online Access:http://dissertations.umi.com/gsas.harvard:10777
http://nrs.harvard.edu/urn-3:HUL.InstRepos:11156813