Elucidating the chemical and thermal unfolding profiles of organophosphorus hydrolase and increasing its operational stability
Organophosphorus hydrolase (OPH, EC 3.1.8.1) is a homodimeric enzyme that has been observed to unfold via a three-state unfolding pathway (N2* ↔ I2 ↔ 2U) under chemical denaturing conditions. The dimeric intermediate (I2) is catalytically inactive and, although this enzyme has a very large overall c...
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| Format: | Others |
| Language: | en_US |
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2010
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| Online Access: | http://hdl.handle.net/1969.1/ETD-TAMU-1237 http://hdl.handle.net/1969.1/ETD-TAMU-1237 |