Phage display selection of recombinant antibodies derived from a chicken immune library against cryopreserved Eimeria tenella sporozoites
An antibody library against Eimeria tenella sporozoites was constructed by phage display. Total RNA was isolated from the spleen, bone marrow, and ceca of immune chickens, and was used to reverse-transcribe cDNA. Heavy and light antibody variable genes were amplified from cDNA by the Polymerase Chai...
Main Author: | |
---|---|
Other Authors: | |
Format: | Others |
Language: | en_US |
Published: |
2010
|
Subjects: | |
Online Access: | http://hdl.handle.net/1969.1/ETD-TAMU-1847 http://hdl.handle.net/1969.1/ETD-TAMU-1847 |