Pyridoxal Phosphate as a Tag to Identify Enzymes Within the “PLP-ome”
The main objective of this research was to develop a protocol in which pyridoxal phosphate (PLP) would act as a tag to identify PLP-dependent enzymes from complex mixtures or cell lysates. Following the purification of a PLP-dependent enzyme (CysM), a method was developed to reduce the PLP-lysine S...
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Format: | Others |
Language: | en_US |
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2012
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Online Access: | http://hdl.handle.net/1969.1/ETD-TAMU-2011-05-9260 |