Pyridoxal Phosphate as a Tag to Identify Enzymes Within the “PLP-ome”

The main objective of this research was to develop a protocol in which pyridoxal phosphate (PLP) would act as a tag to identify PLP-dependent enzymes from complex mixtures or cell lysates. Following the purification of a PLP-dependent enzyme (CysM), a method was developed to reduce the PLP-lysine S...

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Bibliographic Details
Main Author: Messer, Kayla J.
Other Authors: Begley, Tadhg
Format: Others
Language:en_US
Published: 2012
Subjects:
PLP
Online Access:http://hdl.handle.net/1969.1/ETD-TAMU-2011-05-9260