Site Directed Mutagenesis of Dienelactone Hydrolase
The clcD gene encoding dienelactone hydrolase (DLH) is part of the clc gene cluster for the utilization of the B-ketoadipate pathway intermediate chlorocatechol. The roles that individual amino acids residues play in catalysis and binding of the enzyme were investigated. Using PCR a 1.9 kbp clcD fra...
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Format: | Others |
Language: | English |
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University of North Texas
1995
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Online Access: | https://digital.library.unt.edu/ark:/67531/metadc277940/ |