Site Directed Mutagenesis of Dienelactone Hydrolase

The clcD gene encoding dienelactone hydrolase (DLH) is part of the clc gene cluster for the utilization of the B-ketoadipate pathway intermediate chlorocatechol. The roles that individual amino acids residues play in catalysis and binding of the enzyme were investigated. Using PCR a 1.9 kbp clcD fra...

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Bibliographic Details
Main Author: Al-Khatib, Haifa Yousef
Other Authors: Shanley, Mark Stephen
Format: Others
Language:English
Published: University of North Texas 1995
Subjects:
Online Access:https://digital.library.unt.edu/ark:/67531/metadc277940/